Coding
Part:BBa_K209007:Design
Designed by: Ryan Quan Group: iGEM09_UCSF (2009-09-11)
Aar1 C-D part, PI3K1 (full length) (Dictyostelium discoideum)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3160
Illegal SpeI site found at 118
Illegal SpeI site found at 1234
Illegal PstI site found at 3308 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3160
Illegal SpeI site found at 118
Illegal SpeI site found at 1234
Illegal PstI site found at 3308 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3160
Illegal BamHI site found at 4161 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3160
Illegal SpeI site found at 118
Illegal SpeI site found at 1234
Illegal PstI site found at 3308 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3160
Illegal SpeI site found at 118
Illegal SpeI site found at 1234
Illegal PstI site found at 3308 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Aar1 CD part in pDONR221. BBF RFC 28.
Source
from plasmid